Effects of Sodium Chloride on Earthworm Populations
Holly Elsberry, Marie Nikolay, Marissa Van Dyke

Index
Introduction
Methods

Results
Conclusion


Introduction

Sodium Chloride is used as road salt to de-ice the Wisconsin roads in the winter season. Excess use of sodium chloride can seep into the soil and become harmful to the Lake Wingra watershed.
Sodium chloride concentrations can affect animals living in the soil, such as earthworms.
The purpose of this study was to determine if sodium chloride concentrations have an effect on earthworm population on the Edgewood Campus.
Earthworms can be used as a measure of the health of the soil. They aerate the soil and break up organic material and also aid in the decomposition of plant litter, such as the thatch layer, and in recycling of nutrients (Townsend, Potter, & Powell, 2003).
In order to determine the health of the Lake Wingra Watershed we compared earthworm populations on the Edgewood Campus in relation to the sodium chloride concentrations of the earthworm’s habitat.

Methods

Four locations were tested on the Edgewood College Campus; the Dericci Courtyard, Raingarden A (behind the Edgedome), Raingarden B (in front of the Edgedome), and behind Regina near the walkway.
A mustard slurry was used to extract the earthworms from the soil in order to measure the population.
Core samples of soil were taken at each location. Core samples were separated into top soil samples and bottom soil samples.
We assessed the type of soil taken from each of the four sites by using a standard procedure for soil type identification (Environmental Concern, 1995).
Sodium chloride concentrations were obtained by using the Hach Chloride Test Kit: Model 8-P low range 0-100 mg/L.  We converted our results mg/L into mg of chloride per gram of dry weight soil so we could determine the ratio of chloride to soil.
Soil conductivity was obtained through a handheld conductivity device.

Results

Soil types ranged from clay loam to sandy loam.
Chloride concentrations ranged from 33.3 - 166.7 mg/g and conductivity ranged from 38.5 µS/g - 249 µS/g.
There was a strong correlation between each location’s conductivity and its chloride concentration.
There were fewer earthworms where there were higher concentrations of chloride, and areas with lower concentrations had larger earthworm populations
The association of fewer earthworms and higher chloride concentrations was also shown when we averaged each location’s top and bottom soil samples .


Figure 1. Chloride concentrations of each test location in relation to respective conductivity. T (Top Soil); B (Bottom Soil)


Figure 2. Earthworm counts as a function of Chloride concentrations from the top and bottom 1.5 inch core sample of soil measured mg/g

Figure 3. Earthworm counts as a function of the average Chloride concentrations from the top and bottom core samples of each test location

Figure 4. Top and bottom core sample averages of chloride and conductivity

Conclusion

  Results indicate the lack of earthworms in the soil of certain locations may be due to high concentrations of chloride. Locations with lower populations of earthworms had higher sodium chloride concentrations and locations with higher populations of earthworms had lower sodium chloride concentrations. The absence of earthworms in the soil results a determinant of soil quality and health as earthworms serve to aerate the soil and break up plant litter. The use of road salt by the City Madison and the Edgewood College Campus contributes to the sodium chloride concentrations on campus and in the Lake Wingra Watershed.
We reccomend that the City of Madison and the Edgewood College Campus limit the amount of road salt applies to city streets, roads and sidewalks in an effort to protect the soil quality and health of the Lake Wingra Watershed.




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